The ARHGEF12 Polyclonal Antibody (PAC055278) is a valuable tool for researchers studying ARHGEF12, a Rho guanine nucleotide exchange factor involved in cell signaling and cytoskeletal dynamics. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting ARHGEF12 in human samples, making it suitable for Western blot applications.ARHGEF12 is a key regulator of Rho GTPases, which play essential roles in processes such as cell migration, cell adhesion, and cell cycle progression. Dysregulation of ARHGEF12 has been implicated in various diseases, including cancer and neurological disorders.
By enabling the detection and analysis of ARHGEF12 expression, this antibody facilitates research into the mechanisms underlying these conditions.Investigating the functions of ARHGEF12 can provide valuable insights into cellular processes and disease mechanisms, offering potential targets for therapeutic intervention. The ARHGEF12 Polyclonal Antibody is a reliable tool for studying the role of ARHGEF12 in physiological and pathological conditions, making it a valuable asset for researchers in the fields of cell biology and molecular medicine.
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO55278 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO55278 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human testis tissue using PACO55278 at dilution of 1:100.
Background:
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
ARHGEF12: a widely-expressed guanine nucleotide exchange factor (GEF) for RhoA GTPase. May act as GTPase-activating protein (GAP) for G-alpha 12 and 13. The fusion protein MLL-ARHGEF12 appears to be a disease driver in acute myeloid leukemia. Two isoforms of the human protein are produced by alternative splicing.Protein type: GEF; GEF, Rac/Rho; Motility/polarity/chemotaxis; OncoproteinChromosomal Location of Human Ortholog: 11q23.3Cellular Component: cytoplasm; cytosolMolecular Function: G-protein-coupled receptor binding; guanyl-nucleotide exchange factor activity; protein binding; Rho guanyl-nucleotide exchange factor activityBiological Process: G-protein coupled receptor protein signaling pathway; positive regulation of apoptosis; regulation of small GTPase mediated signal transduction
UniProt Protein Details:
NCBI Summary:
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
