The ARX Antibody (CAB17267) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ARX protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in neuroscience and developmental biology.ARX is a crucial factor in neuronal development, playing a significant role in the formation of the brain's cortex and in the regulation of gene expression in neurons. Mutations in the ARX gene have been linked to several neurological disorders, including X-linked lissencephaly and West syndrome.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARX Antibody
SKU:
CAB17267
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
LLRQ PTPA VEGA VASG ALAD PATA AADR RASS IAAL RLKA KEHA AQLT QLNI LPGT STGK EVC
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
SH-SY5Y, U-87MG, Mouse brain, Mouse testis, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
58kDa
Observed MW:
58kDa
This gene is a homeobox-containing gene expressed during development. The expressed protein contains two conserved domains, a C-peptide (or aristaless domain) and the prd-like class homeobox domain. It is a member of the group-II aristaless-related protein family whose members are expressed primarily in the central and/or peripheral nervous system. This gene is thought to be involved in CNS development. Expansion of a polyalanine tract and other mutations in this gene cause X-linked cognitive disability and epilepsy.
Purification Method
Affinity purification
Gene ID
170302
RRID
AB_2768464
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ARX Rabbit pAb (CAB17267) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using ARX Rabbit pAb (CAB17267) at dilution of 1:50 (20x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of C6 cells using ARX Rabbit pAb (CAB17267) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
