The ASAH2 Antibody (CAB12596) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications such as Western blotting. By binding specifically to the ASAH2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in lipid metabolism and related fields.ASAH2, also known as acid ceramidase, plays a crucial role in regulating cellular levels of ceramide, a bioactive lipid involved in processes such as apoptosis, inflammation, and cellular signaling.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ASAH2 Antibody
SKU:
CAB12596
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HNAC1, BCDase, LCDase, NCDase, N-CDase, ASAH2
Positive Sample:
22Rv1, Mouse kidney, Rat kidney
Cellular Localization:
Cell Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
86kDa
Observed MW:
85kDa
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).
Purification Method
Affinity purification
Gene ID
56624
RRID
AB_2759438
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ASAH2 Rabbit pAb (CAB12596) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
