Anti-Neutral ceramidase Antibody (CAB7985)

Antibody Name:	ASAH2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB7985
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human ASAH2

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse heart

Immunogen:	Recombinant protein of human ASAH2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	56624
Uniprot:	Q9NR71
Cellular Location:	Cell membrane, Single-pass type II membrane protein
Calculated MW:	81kDa/85kDa
Observed MW:	100kDa

Synonyms:

ASAH2, BCDase, HNAC1, LCDase, N-CDase, NCDase

Background:

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]

UniProt Protein Function:	ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Lipid Metabolism - sphingolipid; EC 3.5.1.23; Hydrolase; Membrane protein, integral Chromosomal Location of Human Ortholog: 10q11.21 Cellular Component: mitochondrion Biological Process: ceramide metabolic process; signal transduction
NCBI Summary:	Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9NR71
NCBI GenInfo Identifier:	110832757
NCBI Gene ID:	56624
NCBI Accession:	Q9NR71.2
UniProt Secondary Accession:	Q9NR71,Q3KNU1, Q5SNT7, Q5SZP6, Q5SZP7, Q5T1D5, Q71ME6
UniProt Related Accession:	Q9NR71
Molecular Weight:	81,718 Da
NCBI Full Name:	Neutral ceramidase
NCBI Synonym Full Names:	N-acylsphingosine amidohydrolase 2
NCBI Official Symbol:	ASAH2
NCBI Official Synonym Symbols:	HNAC1; BCDase; LCDase; NCDase; N-CDase
NCBI Protein Information:	neutral ceramidase
UniProt Protein Name:	Neutral ceramidase
UniProt Synonym Protein Names:	Acylsphingosine deacylase 2; BCDase; LCDase; hCD
Protein Family:	Neutral ceramidase
UniProt Gene Name:	ASAH2
UniProt Entry Name:	ASAH2_HUMAN

	Western blot analysis of extracts of various cell lines, using ASAH2 Rabbit pAb (CAB7985) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse brain using ASAH2 antibody (CAB7985) (40x lens).
	Immunohistochemistry of paraffin-embedded rat liver using ASAH2 antibody (CAB7985) (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using ASAH2 antibody (CAB7985) (40x lens).

Antibodies

Anti-ASAH2 Antibody (CAB12596)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)