The ASCL4 Antibody (CAB14439) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, exhibits high specificity for human samples and has been validated for use in various applications, including Western blotting.ASCL4, also known as Achaete-scute homolog 4, plays a crucial role in the development of the nervous system by regulating the differentiation of neural progenitor cells. Its involvement in neurogenesis makes it a promising target for research in neurobiology and developmental biology.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ASCL4 Antibody
SKU:
CAB14439
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
METR KPAE RLAL PYSL RTAP LGVP GTLP GLPR RDPL RVAL RLDA ACWE WARS GCAR GWQY LPVP LDSA FE
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:5000
IHC-P
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASH-4, HASH4, bHLHa44, ASCL4
Positive Sample:
Mouse brain, Mouse kidney, Rat liver
Cellular Localization:
Nucleus.
Calculated MW:
19kDa
Observed MW:
19kDa
Basic helix-loop-helix transcription factors, such as ASCL4, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).
Purification Method
Affinity purification
Gene ID
121549
RRID
AB_2761309
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ASCL4 Rabbit pAb (CAB14439) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ASCL4 Rabbit pAb (CAB14439) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using ASCL4 Rabbit pAb (CAB14439) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
