The ATG3 Monoclonal Antibody (CAB19594) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. RRID AB_2862687 Gene ID 64422 Swiss Prot Synonym APG3; APG3L; hApg3; PC3-96; APG3-LIKE; ATG3
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ATG3 Monoclonal Antibody
SKU:
CAB19594
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0073
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IHC-P
1:200 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APG3, APG3L, hApg3, PC3-96, APG3-LIKE, ATG3
Positive Sample:
Jurkat, U-87 MG, K-562, Mouse brain, Mouse testis, Rat testis
Cellular Localization:
Cytoplasm.
Calculated MW:
40kDa
Observed MW:
40kDa
This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. RRID AB_2862687 Gene ID 64422 Swiss Prot Synonym APG3; APG3L; hApg3; PC3-96; APG3-LIKE; ATG3
Purification Method:
Affinity purification
Gene ID:
64422
RRID:
AB_2862687
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATG3 Rabbit mAb (CAB19594) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 40s.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATG3 Rabbit mAb (CAB19594) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
