The ATG3 Antibody (CAB5809) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. RRID AB_2766561 Gene ID 64422 Swiss Prot Synonym APG3; APG3L; hApg3; PC3-96; APG3-LIKE; ATG3
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ATG3 Antibody
SKU:
CAB5809
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APG3, APG3L, hApg3, PC3-96, APG3-LIKE, ATG3
Positive Sample:
K-562, U-87MG, Mouse brain, Mouse kidney, Rat testis
Cellular Localization:
Cytoplasm.
Calculated MW:
36kDa
Observed MW:
40kDa
This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. RRID AB_2766561 Gene ID 64422 Swiss Prot Synonym APG3; APG3L; hApg3; PC3-96; APG3-LIKE; ATG3
Purification Method:
Affinity purification
Gene ID:
64422
RRID:
AB_2766561
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATG3 Rabbit pAb (CAB5809) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of NIH-3T3 cells using ATG3 Rabbit pAb (CAB5809) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
