The ATG4B Antibody (CAB2837) is a high-quality antibody developed for reliable detection and analysis of target proteins. Autophagy is a cellular mechanism that clears out damaged or unnecessary components, playing a crucial role in maintaining cellular health and preventing diseases like cancer and neurodegenerative disorders.This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ATG4B protein, this antibody allows for the detection and analysis of ATG4B in various cell types, providing valuable insights into the autophagy pathway.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATG4B Antibody
SKU:
CAB2837
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APG4B, AUTL1, HsAPG4B, ATG4B
Positive Sample:
RD
Cellular Localization:
Cytoplasm.
Calculated MW:
44kDa
Observed MW:
48kDa
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method
Affinity purification
Gene ID
23192
RRID
AB_2764667
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATG4B Rabbit pAb (CAB2837) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Western blot analysis of lysates from RD cells using ATG4B Rabbit pAb (CAB2837) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
