Description
ATP5A1 Polyclonal Antibody (CAB5884)
The ATP5A1 Polyclonal Antibody (CAB5884) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ATP5A1 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component of studies in bioenergetics, metabolism, and mitochondrial function.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | ATP5A1 Polyclonal Antibody |
| SKU: | CAB5884 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | MLSV RVAA AVVR ALPR RAGL VSRN ALGS SFIA ARNF HASN THLQ KTGT AEMS SILE ERIL GADT SVDL EETG RVLS IGDG IARV HGLR NVQA EEMV EFSS GLKG MSLN LEPD NVGV VVFG NDKL IKEG DIVK RTGA IVDV PVGE ELLG RVVD ALGN AIDG KGPI GSKT RRRV GLKA PGII PRIS VREP MQTG IKAV DSLV PIGR GQRE LIIG DRQT GKTS IAID TIIN QKRF NDGS DEKK KLYC IYVA IGQK RSTV AQLV KRLT DADA MKYT IVVS ATAS | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | OMR, ORM, ATPM, MOM2, ATP5A, hATP1, ATP5A1, MC5DN4, ATP5AL2, COXPD22, HEL-S-123m |
| Positive Sample: | BT-474, SK-OV-3, HeLa, MCF7, Mouse kidney, Mouse heart, Rat kidney, Rat heart |
| Cellular Localization: | Cell Membrane, Extracellular Side, Mitochondrion Inner Membrane, Peripheral Membrane Protein. |
| Calculated MW: | 60kDa |
| Observed MW: | 53kDa |
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
| Purification Method | Affinity purification |
| Gene ID | 498 |
| RRID | AB_2766632 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using ATP5A1 Rabbit pAb (CAB5884) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
 | Confocal immunofluorescence analysis of Hela cells using ATP5A1 Rabbit pAb (CAB5884) at dilution of 1:200. Blue: DAPI for nuclear staining. |
