Description
ATP6V0C Monoclonal Antibody (CAB23757)
The ATP6V0C Monoclonal Antibody (CAB23757) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced through hybridoma technology, boasts superior specificity and sensitivity for the detection of ATP6V0C in various sample types.ATP6V0C is a key component of the vacuolar ATPase complex, essential for maintaining cellular pH homeostasis and regulating vesicular trafficking. Dysregulation of ATP6V0C has been implicated in various diseases, including cancer, osteoporosis, and renal diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | ATP6V0C Monoclonal Antibody |
| SKU: | CAB23757 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Clone Number: | ARC61235 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | ATP6V0C, ATP6C, ATP6L, ATPL, VATL, VPPC, Vma3, ATPase H+ transporting V0 subunit c |
| Positive Sample: | MCF7, 293F |
| Cellular Localization: | Multi-Pass Membrane Protein, Vacuole Membrane. |
| Calculated MW: | 15kDa |
| Observed MW: | 16kDa |
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17.
| Purification Method | Affinity purification |
| Gene ID | 527 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates, using ATP6V0C Rabbit mAb (CAB23757) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
