The ATRIP Antibody (CAB7139) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody was raised in rabbits and exhibits high reactivity with human samples, making it an excellent choice for Western blot applications. By binding specifically to the ATRIP protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in DNA repair, cell cycle regulation, and cancer research.ATRIP is a well-known sensor protein that plays a central role in activating the ATR kinase pathway in response to DNA damage.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ATRIP Antibody
SKU:
CAB7139
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATRIP
Positive Sample:
HepG2, Jurkat, HeLa, Mouse brain, Mouse heart
Cellular Localization:
Nucleus.
Calculated MW:
86kDa
Observed MW:
86kDa
This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
84126
RRID
AB_2767694
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATRIP Rabbit pAb (CAB7139) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
