The BACE1 Monoclonal Antibody (CAB11533) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting BACE1 in human samples, making it suitable for use in various research applications, including Western blotting, immunofluorescence, and immunohistochemistry.BACE1, also known as beta-secretase, is involved in the cleavage of amyloid precursor protein, leading to the production of beta-amyloid peptides that accumulate in the brains of Alzheimer's patients.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BACE1 Monoclonal Antibody
SKU:
CAB11533
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0696
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASP2, BACE, HSPC104, BACE1
Positive Sample:
SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:
Cell Surface, Cytoplasmic Vesicle Membrane, Endoplasmic Reticulum, Endosome, Golgi Apparatus, Membrane, Single-Pass Type I Membrane Protein, Trans-Golgi Network.
Calculated MW:
56kDa
Observed MW:
70kDa
This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients.
Purification Method
Affinity purification
Gene ID
23621
RRID
AB_2861590
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BACE1 Rabbit mAb (CAB11533) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using BACE1 Rabbit mAb (CAB11533) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
