Description
BBC3 Antibody (PACO63199)
The BBC3 Polyclonal Antibody (PAC063199) is a valuable tool for researchers studying the BBC3 protein, also known as Puma, which plays a critical role in the regulation of apoptosis or programmed cell death. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding specifically to the BBC3 protein, this antibody enables detection and analysis in a variety of cell types, making it an essential reagent for studies in cell biology, cancer research, and drug development.BBC3/Puma is a key player in the apoptotic pathway, acting as a pro-apoptotic protein that plays a pivotal role in triggering cell death in response to various cellular stresses.
Dysregulation of the BBC3 gene has been implicated in the development and progression of cancer, making it an attractive target for therapeutic interventions. Understanding the function of BBC3 is crucial for unraveling its role in cancer biology and identifying novel strategies for cancer treatment. Overall, the BBC3 Polyclonal Antibody is a valuable tool for researchers investigating the molecular mechanisms of apoptosis and its implications in cancer biology and drug discovery.
| Antibody Name: | BBC3 Antibody (PACO63199) |
| Antibody SKU: | PACO63199 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Peptide sequence from Human Bcl-2-binding component 3 protein (5-23AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, U87 whole cell lysate, Rat kidney tissue. All lanes: BBC3 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 21, 15 kDa. Observed band size: 21 kDa. |
| Background: | Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis. |
| Synonyms: | Bcl-2-binding component 3 (JFY-1) (p53 up-regulated modulator of apoptosis), BBC3, PUMA |
| UniProt Protein Function: | PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol Molecular Function:protein binding Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth |
| NCBI Summary: | This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q9BXH1 |
| NCBI GenInfo Identifier: | 56748610 |
| NCBI Gene ID: | 27113 |
| NCBI Accession: | Q9BXH1.1 |
| UniProt Secondary Accession: | Q9BXH1,O00171, Q96PG9, B9EGI3, |
| UniProt Related Accession: | Q96PG8,Q9BXH1 |
| Molecular Weight: | 193 |
| NCBI Full Name: | Bcl-2-binding component 3 |
| NCBI Synonym Full Names: | BCL2 binding component 3 |
| NCBI Official Symbol: | BBC3Â Â |
| NCBI Official Synonym Symbols: | JFY1; PUMA; JFY-1Â Â |
| NCBI Protein Information: | bcl-2-binding component 3; p53 up-regulated modulator of apoptosis |
| UniProt Protein Name: | Bcl-2-binding component 3 |
| UniProt Synonym Protein Names: | JFY-1; p53 up-regulated modulator of apoptosis |
| Protein Family: | Bcl-2-binding component |
| UniProt Gene Name: | BBC3Â Â |
| UniProt Entry Name: | BBC3_HUMAN |
| Antibodies | ELISA Kits |
| BBC3 Antibody (PACO01380) | Human PUMA (P53 Upregulated Modulator of Apoptosis) CLIA Kit (HUES00816) |
| Human Bcl-2-binding component 3 (BBC3) ELISA Kit |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
