The CTIP1/BCL11A Monoclonal Antibody (CAB9231) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using innovative technology, delivers high specificity and sensitivity in detecting CTIP1 in human samples. Validated for use in Western blot and immunofluorescence applications, this antibody enables precise visualization and analysis of CTIP1 expression in different cell types.CTIP1, also known as RBBP8, is crucial for maintaining genomic stability and regulating gene expression.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
CTIP1/BCL11A Monoclonal Antibody
SKU:
CAB9231
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1486
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EVI9, CTIP1, DILOS, ZNF856, HBFQTL5, SMARCM1, 1A
Positive Sample:
293T, Rat brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
91kDa
Observed MW:
120kDa
This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
53335
RRID
AB_2863692
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat brain using [KO Validated] CTIP1/BCL11A Rabbit mAb (CAB9231) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from wild type(WT) and CTIP1/BCL11A knockout (KO) 293T(KO) cells, using CTIP1/BCL11A Rabbit mAb (CAB9231) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
