Description
| Product Name: | Bet v 1 ELISA 2.0 EP kit - Single plate pack |
| SKU: | SEIB00010 |
| Antibodies: | 5B4 / 6H4 |
| Standard Range: | 1.56-100ng/mL |
| Limit of Detection: | 0.39 ng/mL |
| Background: | OD < 0.08 at 450 nm |
| Coefficient of Determination: | R² > 0.98 |
| Contents: |
Plate: Pre-coated with anti-Bet v 1 monoclonal antibody 5B4 Vial 1 (white top): Bet v 1 allergen standard (1000 ng/mL) Vial 2 (brown): Biotinylated monoclonal antibody 6H4 Vial 3 (blue top): Streptavidin-peroxidase Bottle 1: Wash buffer (10× concentrate) Bottle 2: Assay buffer (10× concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid) |
| Storage: | Store at 2–8 °C |
| Not Provided: |
Type I ultrapure or 18.2 MΩ de-ionized water Volumetric measuring equipment (serological pipette, cylinder) Clean containers for buffer/reagent preparation Calibrated single & multi-channel micropipettes and tips Vortex mixer Plate reader (450 nm) Analysis software (recommended) |
| Notes: |
The allergen standard is recommended for immunoassay calibration purposes only. FAQs and troubleshooting guide available under the ‘Support’ tab on our website. |
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 4B10 and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
| Monoclonal Antibody: | 5B4 (clone 5B4) |
| Immunogen: | Birch pollen extract |
| Isotype: | Mouse IgG1 |
| Specificity: | Recognizes distinct epitope on Betula verrucosa allergen, Bet v 1. Minimal cross-reactivity with PR-10 family of pollen allergens. |
| Purification: | Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. |
| Detection Antibody: | 6H4 (clone 6H4) |
| Immunogen: | Birch pollen extract |
| Isotype: | Mouse IgG1 |
| Specificity: | Recognizes distinct epitope on Betula verrucosa allergen, Bet v 1. Minimal cross-reactivity with PR-10 family of pollen allergens. |
| Purification: | Produced in tissue culture and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. |
| Biotinylation: | Biotinylated and titrated for ELISA use at 1/1000 dilution. Prepared in 1% BSA/50% glycerol-PBS, pH 7.4, 0.22 μm filtered, preservative-free. |
| Allergen Standard: | Recombinant Bet v 1a prepared in 1% BSA / 50% glycerol-PBS, pH 7.4. |
| Concentration: | 1000 ng/mL (Substandardized against the European Pharmacopoeia rBet v 1 CRS, Y0001565) |
