The BH2 (Dihydrobiopterin) ELISA Kit is specifically designed for the precise measurement of BH2 levels in various samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for a variety of research purposes.BH2 is a key molecule involved in the regulation of nitric oxide synthesis and plays a critical role in various physiological processes, including cardiovascular function and immune response.
Dysregulation of BH2 levels has been linked to conditions such as cardiovascular diseases, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these diseases and exploring potential treatment options.Overall, the BH2 ELISA Kit is a reliable tool for researchers seeking to investigate the role of BH2 in health and disease, providing valuable insights into its significance in various biological pathways.
Product Name:
BH2 (Dihydrobiopterin) ELISA Kit
SKU:
UNES00060
Target:
BH2 (Dihydrobiopterin) ELISA Kit (UNES00060)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BH2. During the reaction, BH2 in the sample or standard competes with a fixed amount of BH2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BH2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BH2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
85-100
98-113
Average (%)
93
91
106
1:4
Range (%)
84-99
88-101
100-118
Average (%)
91
94
108
1:8
Range (%)
85-95
80-93
100-113
Average (%)
90
87
107
1:16
Range (%)
87-101
85-96
98-111
Average (%)
94
91
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
100
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.11
316.37
963.82
106.55
327.65
982.41
Standard deviation
7.22
15.91
48.87
7.06
16.64
35.76
C V (%)
6.56
5.03
5.07
6.63
5.08
3.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of BH2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes BH2 in samples. No significant cross-reactivity or interference between BH2 and analogues was observed.
