The BGN Antibody (CAB5770) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications, making it an ideal choice for investigations in the fields of biology and medicine.Biglycan is known to interact with various growth factors, cytokines, and receptors, influencing processes such as cell adhesion, migration, proliferation, and differentiation. Its dysregulation has been associated with various diseases, including cancer, cardiovascular disorders, and musculoskeletal conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
BGN Antibody
SKU:
CAB5770
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in bone growth, muscle development and regeneration, and collagen fibril assembly in multiple tissues. This protein may also regulate inflammation and innate immunity. Additionally, the encoded protein may contribute to atherosclerosis and aortic valve stenosis in human patients. This gene and the related gene decorin are thought to be the result of a gene duplication.
Purification Method
Affinity purification
Gene ID
633
RRID
AB_2766524
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BGN using BGN Rabbit pAb (CAB5770) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HCT 116 cells using BGN Rabbit pAb(CAB5770) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
