Biotinylated Recombinant Human Fc gamma RIIA/FCGR2A/CD32a (H167R) Protein
The Biotinylated Recombinant Human Fc gamma RIIA/FCGR2A/CD32a (H167R) Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated Fc gamma RIIA/FCGR2A/CD32a (H167R) function and its implications in health and disease.
This product (SKU: RPCB0626) is produced using advanced expression systems and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 23.2 kDa with an observed molecular weight of 30-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human Fc gamma RIIA/FCGR2A/CD32a (H167R) Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human Fc gamma RIIA/FCGR2A/CD32a (H167R) Protein (RPCB0626), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (10 -8 -10 -9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (10 -6 -10 -7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Biotinylated Recombinant Human Fc gamma RIIA/FCGR2A/CD32a (H167R) Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Fc gamma RIIA (R167) is greater than 95% as determined by SEC-HPLC.
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (R167), His-Avi Tag with an affinity constant of 1.03 μM as determined in SPR assay (Biacore T200).
