Biotinylated Recombinant Human HBV (HLA-A*02:01) Protein
The Biotinylated Recombinant Human HBV (HLA-A*02:01) Protein is a specially modified recombinant protein featuring biotin conjugation for enhanced detection and purification capabilities. This biotinylated variant is particularly valuable in immunoassay development, protein interaction studies, flow cytometry applications, and biosensor technologies, offering researchers improved sensitivity and specificity in experimental workflows requiring streptavidin-biotin binding systems.
This product (SKU: RPCB1770) is produced using HEK293 cells and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 50.5 kDa with an observed molecular weight of 52-62 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human HBV (HLA-A*02:01) Protein
SKU:
RPCB1770
Size:
100 μg
Reactivity:
Human
Synonyms:
Hepatitis B virus, MHC, HBV
Tag:
C-His&Avi
Expression Host:
HEK293 cells
Calculated MW:
50.5 kDa
Observed MW:
52-62 kDa
Protein Description:
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human HBV(HLA-A*02:01) Protein (RPCB1770), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Hepatitis B virus (HBV), is the leading cause of liver diseases infecting an estimated 240 million persons worldwide. The HBV prevalence rates are variables between different countries, with an high level of endemicity in the south-eastern part of Europe. Seven main HBV-D subgenotypes have been described until now (D1-D7).
Biotinylated Recombinant Human HBV(HLA-A*02:01) Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human HBV (HLA-A*02:01) is greater than 95% as determined by SEC-HPLC.
