Biotinylated Recombinant Human HLA-G Complex Protein
The Biotinylated Recombinant Human HLA-G Complex Protein is a specially modified recombinant protein featuring biotin conjugation for enhanced detection and purification capabilities. This biotinylated variant is particularly valuable in immunoassay development, protein interaction studies, flow cytometry applications, and biosensor technologies, offering researchers improved sensitivity and specificity in experimental workflows requiring streptavidin-biotin binding systems.
This product (SKU: RPCB1774) is produced using HEK293 cells and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 50.5 kDa with an observed molecular weight of 51-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human HLA-G Complex Protein
SKU:
RPCB1774
Size:
100 μg
Reactivity:
Human
Synonyms:
HLA G antigen, sHLA-G, b2 microglobulin, HLA G, HLAG, HLA-G, MHC Class I Antigen G, MHC class Ib antigen, MHC-G, sHLA-G
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human HLA-G Complex Protein (RPCB1774), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Biotinylated Recombinant Human HLA-G Complex Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human HLA-G is greater than 95% as determined by SEC-HPLC.
