Description
Biotinylated Recombinant Human TNF-alpha Protein
The Biotinylated Recombinant Human TNF-alpha Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated TNF-alpha function and its implications in health and disease.
This product (SKU: RPCB0042) is produced using advanced expression systems and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 20.3 kDa with an observed molecular weight of 21-24 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | Biotinylated Recombinant Human TNF-alpha Protein |
SKU: | RPCB0042 |
Size: | 100 μg |
Reactivity: | Human |
Synonyms: | TNF, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, TNF-α, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, TNF alpha |
Tag: | C-His&Avi |
Calculated MW: | 20.3 kDa |
Observed MW: | 21-24 kDa |
Gene ID: | 7124 |
Protein Description: | High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human TNF-alpha Protein (RPCB0042), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC. |
Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Storage: | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.