The Bmi1 Monoclonal Antibody (CAB17914) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the BMI1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in stem cell biology, cancer research, and developmental biology.BMI1 is a critical regulator of cell proliferation and differentiation, playing a key role in maintaining the self-renewal capacity of stem cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Bmi1 Monoclonal Antibody
SKU:
CAB17914
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC0376
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCGF4, RNF51, FLVI2/BMI1, flvi-2/bmi-1, Bmi1
Positive Sample:
K-562, Rat testis
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
37kDa
Observed MW:
40kDa
This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
Purification Method
Affinity purification
Gene ID
648
RRID
AB_2861759
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Bmi1 Rabbit mAb (CAB17914) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
