The BNP Monoclonal Antibody (CAB23996) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced in mice, is extremely sensitive and specific for detecting BNP in human samples, making it a valuable tool for diagnostic and research purposes.With its high affinity for BNP, this antibody is ideal for use in immunoassays, ELISA, and Western blot applications to accurately quantify and analyze BNP levels in biological samples. By targeting BNP, researchers can gain insights into cardiac function and pathology, allowing for early detection and monitoring of heart failure and related cardiovascular diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BNP Monoclonal Antibody
SKU:
CAB23996
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC62057
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BNP, Iso-ANP
Positive Sample:
Mouse brain, Mouse liver, Mouse heart, Rat heart, Rat brain
Cellular Localization:
Secreted.
Calculated MW:
15kDa
Observed MW:
15kDa
This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The presence of myocardial injury is a significant predictor of mortality in hospitalized coronavirus disease 2019 (COVID-19) patients, and there is evidence of increased levels of natriuretic peptide B in hospitalized non-survivor COVID-19 patients. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis.
Purification Method
Affinity purification
Gene ID
4879
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using BNP Rabbit mAb (CAB23996) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates, using BNP Rabbit mAb (CAB23996) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
