The Bovine BCL2-associated X protein (Bax) ELISA Kit is a reliable and accurate tool for the quantitative detection of Bax levels in bovine serum, plasma, and cell lysates. This kit is designed with high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Bax is a key regulator of apoptosis, playing a crucial role in programmed cell death. Dysregulation of Bax has been implicated in various diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, measuring Bax levels can provide valuable insights into the mechanisms underlying these conditions and aid in the development of potential therapeutic interventions.With its user-friendly protocol and superior performance, the Bovine Bax ELISA Kit from Assay Genie is an indispensable tool for researchers seeking to investigate the roles of Bax in health and disease.
Product Name:
Bovine Bcl2 Associated X Protein (Bax) ELISA Kit
Product Code:
BODL00020
Size:
96 Assays
Target:
Bax
Synonyms:
BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
