Bovine Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) ELISA Kit
The Bovine Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains Protein 1 (Tie1) ELISA Kit is a powerful tool for the precise measurement of Tie1 levels in bovine samples. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Tie1 is a key protein involved in angiogenesis and vascular development, playing a critical role in regulating blood vessel formation and maintenance. Dysregulation of Tie1 has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions.
As such, Tie1 serves as an important biomarker for studying these diseases and exploring potential therapeutic interventions.The Bovine Tie1 ELISA Kit from Assay Genie provides researchers with a reliable and robust platform for quantifying Tie1 levels in bovine samples. With its high performance and ease of use, this ELISA kit is an indispensable tool for advancing research in the fields of angiogenesis, vascular biology, and beyond.
Product Name:
Bovine Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) ELISA Kit
Product Code:
BODL00168
Size:
96 Assays
Target:
Tie1
Synonyms:
JTK14, Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1, Tyrosine-protein kinase receptor Tie-1
Detection Method:
Sandwich
Range:
31.25-2,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
87-100
93
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
