Description
B-Raf Antibody (CAB15033)
The B-Raf Antibody (CAB15033) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. It specifically targets the BRAF protein, allowing for its detection and analysis in various cell types.BRAF is a protein kinase that is frequently mutated in various cancers, making it a crucial target for cancer research. Mutations in the BRAF gene can lead to uncontrolled cell growth and division, contributing to the development and progression of cancer.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | B-Raf Antibody |
| SKU: | CAB15033 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | DEDH RNQF GQRD RSSS APNV HINT IEPV NIDD LIRD QGFR GDGG STTG LSAT PPAS LPGS LTNV KALQ KSPG PQRE RKSS SSSE DRNR MKTL GRRD SSDD | ||||||
| Tested Applications: | WB IHC-P ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | NS7, B-raf, BRAF1, RAFB1, B-RAF1, BRAF-1, B-Raf |
| Positive Sample: | LO2, U-87MG, HeLa, Jurkat |
| Cellular Localization: | Cell Membrane, Cytoplasm, Nucleus. |
| Calculated MW: | 84kDa |
| Observed MW: | 105kDa |
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
| Purification Method | Affinity purification |
| Gene ID | 673 |
| RRID | AB_2761913 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Immunohistochemistry analysis of paraffin-embedded Rat heart using B-Raf Rabbit pAb (CAB15033) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver using B-Raf Rabbit pAb (CAB15033) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
