Description
B-Raf Antibody (CAB2988)
The B-Raf Antibody (CAB2988) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with BRAF in human samples and has been validated for use in Western blot applications. By specifically binding to the BRAF protein, researchers can detect and analyze BRAF expression in a variety of cell types, making it a crucial tool for investigations in oncology and molecular biology.BRAF is a critical component of the MAPK/ERK signaling pathway, which regulates cell growth, proliferation, and differentiation. Mutations in the BRAF gene can lead to uncontrolled cell growth and contribute to the development of various malignancies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | B-Raf Antibody |
| SKU: | CAB2988 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | QKPI VRVF LPNK QRTV VPAR CGVT VRDS LKKA LMMR GLIP ECCA VYRI QDGE KKPI GWDT DISW LTGE ELHV EVLE NVPL TTHN FVRK TFFT LAFC DFCR KLLF QGFR CQTC GYKF HQRC STEV PLMC VNYD QLDL LFVS KFFE HHPI PQEE ASLA ETAL T | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
| ||||
| Synonyms: | NS7, B-raf, BRAF1, RAFB1, B-RAF1, BRAF-1, B-Raf |
| Positive Sample: | COS-7, WCH |
| Cellular Localization: | Cell Membrane, Cytoplasm, Nucleus. |
| Calculated MW: | 84kDa |
| Observed MW: | 87kDa |
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
| Purification Method | Affinity purification |
| Gene ID | 673 |
| RRID | AB_2764798 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using B-Raf Rabbit pAb (CAB2988) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
