The BTAF1 Antibody (CAB5811) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the BtaF1 protein, this antibody allows for the detection and analysis of BtaF1 in various cell types, making it an essential asset for studies in microbiology and antimicrobial resistance.BtaF1 is a key player in bacterial metabolism and has been linked to antibiotic resistance mechanisms in bacterial pathogens.
This antibody is validated for use in WB, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
BTAF1 Antibody
SKU:
CAB5811
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
MOT1, TAF172, TAFII170, TAF(II)170, BTAF1
Positive Sample:
HepG2, HeLa, MCF7, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
207kDa
Observed MW:
250kDa
This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.
Purification Method
Affinity purification
Gene ID
9044
RRID
AB_2766563
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BTAF1 Rabbit pAb (CAB5811) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
Immunoprecipitation analysis of 150 μg extracts of HeLa cells using 3 μg BTAF1 antibody (CAB5811). Western blot was performed from the immunoprecipitate using BTAF1 antibody (CAB5811) at a dilution of 1:500.
