The C3c Polyclonal Antibody (CAB23938) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit immunization, exhibits high reactivity towards human samples and is validated for use in Western blotting applications. By specifically binding to the C3c protein, researchers can accurately detect and analyze its expression in various cell types, making it an invaluable tool for studies in immunology and inflammatory diseases.C3c, a key player in the complement system, plays a vital role in mediating immune responses by promoting inflammation and opsonization.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human samples.
Product Name:
C3c Polyclonal Antibody
SKU:
CAB23938
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
Purification Method
Affinity purification
Gene ID
718
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells, using C3c Rabbit pAb (CAB23938) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of paraffin-embedded Human liver using C3c Rabbit pAb(CAB23938) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
