The Carbonic Anhydrase 9 (CA9/G250) Antibody (CAB13682) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific for human CA9 samples and is validated for use in applications such as Western blot and immunohistochemistry.CA9, also known as carbonic anhydrase IX, plays a crucial role in regulating pH levels within tumor microenvironments, promoting cancer cell survival and metastasis.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Carbonic Anhydrase 9 (CA9/G250) Antibody
SKU:
CAB13682
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MN, CAIX, Carbonic Anhydrase 9 (CA9/G250)
Positive Sample:
Mouse small intestine, Rat small intestine
Cellular Localization:
Cell Membrane, Cell Projection, Nucleus, Single-Pass Type I Membrane Protein, Microvillus Membrane, Nucleolus.
Calculated MW:
50kDa
Observed MW:
58kDa
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
Purification Method
Affinity purification
Gene ID
768
RRID
AB_2760543
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (CAB13682) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (CAB13682) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human Renal cell carcinoma tissue using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (CAB13682) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human stomach tissue using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (CAB13682) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
