Description
Carbonic Anhydrase 9 (CA9/G250) Antibody (CAB1658)
The Carbonic Anhydrase 9 (CA9/G250) Antibody (CAB1658) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody has high specificity for CA9 in human samples and is validated for use in Western blot applications. By binding to the CA9 protein, this antibody allows for the detection and analysis of CA9 expression in various cell types.CA9 is a transmembrane enzyme that plays a critical role in regulating pH in tumor microenvironments, making it an attractive target for cancer research. High levels of CA9 expression have been associated with poor prognosis in several types of cancer, making it a potential biomarker for disease progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
| Product Name: | Carbonic Anhydrase 9 (CA9/G250) Antibody |
| SKU: | CAB1658 |
| Size: | 20μL, 100μL |
| Reactivity: | Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | GSSG EDDP LGEE DLPS EEDS PREE DPPG EEDL PGEE DLPG EEDL PEVK PKSE EEGS LKLE DLPT VEAP GDPQ EPQN NAHR DKEG DDQS HWRY GGDP PWPR | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | MN, CAIX, Carbonic Anhydrase 9 (CA9/G250) |
| Positive Sample: | Mouse liver, Rat liver |
| Cellular Localization: | Cell Membrane, Cell Projection, Nucleus, Single-Pass Type I Membrane Protein, Microvillus Membrane, Nucleolus. |
| Calculated MW: | 50kDa |
| Observed MW: | 54kDa |
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
| Purification Method | Affinity purification |
| Gene ID | 768 |
| RRID | AB_2763715 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (CAB1658) at 1:100 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
