The CALM3 Antibody (CAB1161) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CALM3 protein, this antibody enables the detection and analysis of CALM3 in different cell types, making it suitable for research in cell biology and neuroscience.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CALM3 Antibody
SKU:
CAB1161
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MADQ LTEE QIAE FKEA FSLF DKDG DGTI TTKE LGTV MRSL GQNP TEAE LQDM INEV DADG NGTI DFPE FLTM MARK MKDT DSEE EIRE AFRV FDKD GNGY ISAA ELRH VMTN LGEK LTDE EVDE MIRE ADID GDGQ VNYE EFVQ MMTA K
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of a family of proteins that binds calcium and functions as a enzymatic co-factor. Activity of this protein is important in the regulation of the cell cycle and cytokinesis. Multiple alternatively spliced transcript variants have been observed at this gene.
Purification Method
Affinity purification
Gene ID
808
RRID
AB_2758634
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse brain, using CALM3 Rabbit pAb (CAB1161). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using CALM3 Rabbit pAb (CAB1161) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
