The Calpain 2 Polyclonal Antibody (CAB2671) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from rabbit immunizations, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blotting applications.Calpain-2 is known for its role in cell signaling, cytoskeletal remodeling, and apoptosis, making it a key player in various physiological and pathological conditions. By targeting the calpain-2 protein, this antibody allows for the detection and analysis of calpain-2 levels in different cell types, offering valuable insights into its function and potential therapeutic applications.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Calpain 2 Polyclonal Antibody
SKU:
CAB2671
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CANP2, mCANP, CANPL2, CANPml, Calpain 2
Positive Sample:
293T, Mouse brain, Mouse skin
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
80kDa
Observed MW:
80kDa
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
824
RRID
AB_2764534
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Calpain 2 Rabbit pAb (CAB2671). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of various lysates using Calpain 2 Rabbit pAb (CAB2671) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Calpain 2 Rabbit pAb (CAB2671) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using Calpain 2 Rabbit pAb (CAB2671) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
