Calpain small subunit 1 Monoclonal Antibody (CAB8859)
The Calpain small subunit 1 Monoclonal Antibody (CAB8859) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. RRID AB_2863618 Gene ID 826 Swiss Prot Synonym CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4; Calpain small subunit 1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Calpain small subunit 1 Monoclonal Antibody
SKU:
CAB8859
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1326
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CANP, CDPS, CSS1, CANPS, CAPN4, CALPAIN4, Calpain small subunit 1
Positive Sample:
293T, A-549, Mouse brain, Rat lung
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
28kDa
Observed MW:
28kDa
This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. RRID AB_2863618 Gene ID 826 Swiss Prot Synonym CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4; Calpain small subunit 1
Purification Method:
Affinity purification
Gene ID:
826
RRID:
AB_2863618
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Calpain small subunit 1 Rabbit mAb (CAB8859) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
