The [KO Validated] Calpain 2 Antibody (CAB1861) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for detecting CAPN2 in human samples and has been validated for use in Western blot applications.By targeting the CAPN2 protein, this antibody enables researchers to investigate the role of CAPN2 in different cell types and physiological conditions. With its high specificity and sensitivity, it is an excellent choice for studies in cell biology, cancer research, and neurodegenerative diseases where CAPN2 dysregulation has been implicated.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] Calpain 2 Antibody
SKU:
CAB1861
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CANP2, mCANP, CANPL2, CANPml, 2
Positive Sample:
HeLa, U-251MG, Mouse kidney, Mouse heart, Rat kidney
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
80kDa
Observed MW:
80kDa
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
824
RRID
AB_2862365
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and Calpain 2 knockout (KO) 293T cells, using [KO Validated] Calpain 2 Rabbit pAb (CAB1861) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
