The CARF Polyclonal Antibody (PACO15413) is a valuable tool for researchers studying CARF, a novel gene involved in cellular function and potential immune regulation. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in various research applications, including Western blot analyses. By binding to the CARF protein, this antibody enables precise detection and analysis in different cell types, making it ideal for investigations in immunology and cancer research.CARF, also known as a potential immune regulator, has been implicated in controlling immune responses and maintaining immune homeostasis.
Research into CARF's functions holds great promise in understanding how it may impact immune-related diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. The CARF Polyclonal Antibody (PACO15413) offers researchers a powerful tool to explore CARF's role in immune regulation and potentially uncover new therapeutic targets for these complex diseases.
Antibody Name:
CARF Antibody (PACO15413)
Antibody SKU:
PACO15413
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human
Immunogen:
Fusion protein of human CARF
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human testis tissue, K562 cells, Primary antibody: PACO15413(CARF Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes.
Background:
CARF (calcium-response factor), also known as ALS2CR8 (amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein) or NYD-SP24, is a 725 amino acid, nuclear protein. Expressed in a wide variety of tissues with highest expression in the hippocampus, CARF is thought to be a transcription factor that associates with the p53 tumor suppression pathway. CARF cooperates, co-localizes and is co-regulated with ARF, an ADP-ribosylation factor, and, through this interaction, helps to mediate ARF-p53-induced apoptotic signaling. This apoptotic pathway is implicated in cell cycle control, proper cellular development, response to DNA damage and the aging process, suggesting that CARF participates in various events throughout the cell.
Synonyms:
calcium responsive transcription factor
UniProt Protein Function:
CARF: May be a transcription factor. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription factorChromosomal Location of Human Ortholog: 2q33.2Cellular Component: nucleusMolecular Function: DNA binding; transcription factor activity
