The Caspase-2 Monoclonal Antibody (CAB4888) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the caspase-2 protein, this antibody enables precise detection and analysis in various cell types, providing valuable insights into apoptosis and its role in disease processes.Caspase-2 is a critical regulator of apoptosis, with implications in cancer, neurodegenerative diseases, and other pathological conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Caspase-2 Monoclonal Antibody
SKU:
CAB4888
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0317
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
GKNH TQSP GCEE SDAG KEEL MKMR LPTR SDMI CGYA CLKG NAAM RNTK RGSW YIEA LTQV FSER ACDM HVAD MLVK VNAL IKER EGYA PGTE FHRC KEMS EYCS TLCQ QLYL FPGY PPT
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
835
RRID
AB_2863374
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Caspase-2 pAb (CAB4888) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) for 3 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
