The Caspase-3 Polyclonal Antibody (CAB11953) is an essential tool for researchers studying apoptosis, the process of programmed cell death. Caspase-3 is a key enzyme in the apoptotic pathway, making this antibody crucial for understanding cell death mechanisms and their implications in various diseases, including cancer and neurodegenerative disorders.Raised in rabbits, this antibody is highly specific to human caspase-3 and has been validated for use in Western blot applications. By binding to caspase-3 protein, researchers can accurately detect and analyze levels of this critical enzyme in different cell types, allowing for in-depth studies in apoptosis and related research areas.
The role of caspase-3 in cell death signaling pathways makes it a promising target for therapeutic interventions aimed at controlling cell survival and death. By utilizing this antibody, researchers can gain valuable insights into the mechanisms of apoptosis and potentially identify new strategies for treating conditions characterized by dysregulated cell death processes.
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
836
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Caspase-3 Rabbit pAb (CAB11953) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
