The Caspase-6 Monoclonal Antibody (CAB24004) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed for use in various research applications, is highly specific to caspase-6 in human samples, providing reliable and accurate results. Caspase-6 is known to play a critical role in various physiological processes, including development, tissue homeostasis, and immune response. Dysregulation of caspase-6 has been implicated in diseases such as neurodegenerative disorders and cancer, highlighting the importance of studying its function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Caspase-6 Monoclonal Antibody
SKU:
CAB24004
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC3212
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCH2, CSP-6, caspase-6, Caspase-6
Positive Sample:
Jurkat, MCF7, RAW 264.7, C6
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
35kDa
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
Purification Method
Affinity purification
Gene ID
839
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Caspase-6 Rabbit mAb (CAB24004) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): THP-1. Exposure time: 20 s.
