The Cyclin D1 Antibody (CAB11022) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.CCND1, also known as cyclin D1, is a key regulator of the G1/S phase transition in the cell cycle, promoting cell division and growth. Dysregulation of CCND1 has been implicated in various cancers, making it an important target for cancer research. By binding specifically to the CCND1 protein, this antibody enables the detection and analysis of CCND1 expression in different cell types, making it ideal for studies in oncology and cell biology.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cyclin D1 Antibody
SKU:
CAB11022
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BCL1, PRAD1, U21B31, D11S287E, Cyclin D1
Positive Sample:
Hep G2, Mouse lung, Rat lung
Cellular Localization:
Cytoplasm, Membrane, Nucleus.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
Purification Method
Affinity purification
Gene ID
595
RRID
AB_2758370
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Hep G2 cells using Cyclin D1 Rabbit pAb (CAB11022) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
Western blot analysis of various lysates using Cyclin D1 Rabbit pAb (CAB11022) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
Immunofluorescence analysis of HeLa cells using Cyclin D1 Rabbit pAb (CAB11022) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg Cyclin D1 Rabbit pAb (CAB11022). Western blot was performed from the immunoprecipitate using Cyclin D1 Rabbit pAb(CAB11022) at a dilution of 1:1000.
