The CD163 Monoclonal Antibody (CAB22619) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal antibody technology, exhibits high specificity and sensitivity for detecting CD163 in various biological samples, particularly in human tissues.CD163 is a scavenger receptor that plays a crucial role in the clearance of hemoglobin-haptoglobin complexes, thereby reducing oxidative stress and inflammation. Its expression on macrophages and monocytes makes it a key player in regulating immune responses and resolving inflammation. The CD163 Monoclonal Antibody is optimized for use in immunohistochemistry and flow cytometry applications, enabling researchers to visualize and quantify CD163 expression in different cell populations.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human samples.
Product Name:
CD163 Monoclonal Antibody
SKU:
CAB22619
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC58325
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
M130, MM130, SCARI1, CD163
Positive Sample:
SH-SY5Y
Cellular Localization:
Cell Membrane, Secreted.
Calculated MW:
125kDa
Observed MW:
170kDa
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
9332
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from SH-SY5Y cells using CD163 Rabbit mAb (CAB22619) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Confocal imaging of paraffin-embedded Human liver tissue using CD163 Rabbit mAb (CAB22619, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
