The CD20 Monoclonal Antibody (CAB4893) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity for human samples, making it ideal for various immunological assays.CD20 is a crucial protein involved in B cell development and function, as well as in immune response regulation. The CD20 Rabbit Monoclonal Antibody (CAB4893) is optimized for use in Western blotting, immunohistochemistry, and flow cytometry applications, enabling researchers to efficiently detect and analyze CD20 expression in different cell types and tissues.
This antibody is validated for use in WB, IHC-P, IF/ICC, FC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD20 Monoclonal Antibody
SKU:
CAB4893
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC51683
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.
Purification Method
Affinity purification
Gene ID
931
RRID
AB_2863378
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD20 Rabbit mAb (CAB4893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Western blot analysis of various lysates, using CD20 Rabbit mAb (CAB4893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HeLa Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD20 Rabbit mAb (CAB4893) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Confocal imaging of Daudi and Jurkat(Negative control) cells using CD20 Rabbit mAb (CAB4893,dilution 1:1000)(Red). DAPI was used for nuclear staining (blue). Objective: 100x.
