The CD20 Monoclonal Antibody (CAB4893) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.
This antibody is validated for use in WB, IHC-P, IF/ICC, FC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD20 Monoclonal Antibody
SKU:
CAB4893
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC51683
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCFCELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IHC-P
1:1000 - 1:10000
IF/ICC
1:800 - 1:3200
FC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.
Purification Method
Affinity purification
Gene ID
931
RRID
AB_2863378
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD20 Rabbit mAb (CAB4893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Western blot analysis of various lysates, using CD20 Rabbit mAb (CAB4893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HeLa Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD20 Rabbit mAb (CAB4893) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Confocal imaging of Daudi and Jurkat(Negative control) cells using CD20 Rabbit mAb (CAB4893,dilution 1:1000)(Red). DAPI was used for nuclear staining (blue). Objective: 100x.
