The CD22 Antibody (CAB5790) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.CD22 is a transmembrane protein found on B cells, where it functions as a negative regulator of B-cell receptor signaling, inhibiting excessive activation and promoting immune tolerance. Research into CD22 has shown its involvement in autoimmune diseases, B-cell lymphomas, and other immune-related disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD22 Antibody
SKU:
CAB5790
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SIGLEC2, SIGLEC-2, CD22
Positive Sample:
Raji, Mouse thymus, Rat lung
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
95kDa
Observed MW:
140kDa
Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.
Purification Method
Affinity purification
Gene ID
933
RRID
AB_2766542
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using CD22 Rabbit pAb (CAB5790) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using CD22 Rabbit pAb (CAB5790) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
