The PE-Cyanine5 Anti-Human CD274 (PD-L1) Antibody (29E.2A3) available at assaygenie.com is a vital tool for researchers studying immune checkpoint proteins in the context of cancer and immunotherapy. This antibody, specifically designed for detecting CD274 (also known as PD-L1), is highly reactive with human samples, enabling precise and reliable analysis in various experimental settings.PD-L1 is a crucial immune checkpoint protein that plays a significant role in regulating immune responses and promoting immune evasion by cancer cells. Understanding the expression and function of PD-L1 is essential for developing novel cancer therapies targeting the PD-1/PD-L1 axis.
The PE-Cyanine5 Anti-Human CD274 (PD-L1) Antibody (29E.2A3) is validated for use in flow cytometry and other experimental techniques, allowing researchers to accurately assess PD-L1 expression levels on cells of interest.By using this antibody, researchers can delve into the complexities of immune checkpoint regulation and explore potential therapeutic strategies aimed at modulating the immune response in cancer and other diseases. The specificity and reliability of the PE-Cyanine5 Anti-Human CD274 (PD-L1) Antibody (29E.2A3) make it an invaluable asset for advancing research in immunology, oncology, and beyond.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
Q9NZQ7
Gene ID:
29126
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.
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