The CD31/PECAM1 Monoclonal Antibody (CAB19014) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
This antibody is validated for use in WB, IHC-P, ELISA, IF-F, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD31/PECAM1 Monoclonal Antibody
SKU:
CAB19014
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC50362
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-FIF-P
Recommended Dilution:
WB
1:5000 - 1:13000
IF-F
1:200 - 1:600
IF-P
1:200 - 1:600
IHC-P
1:500 - 1:3000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Junction, Cell Junction, Cell Membrane, Lipid-Anchor, Single-Pass Type I Membrane Protein.
Calculated MW:
83kDa
Observed MW:
135kDa
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Purification Method
Affinity purification
Gene ID
5175
RRID
AB_2862506
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CD31/PECAM1 Rabbit mAb (CAB19014) at 1:12500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): SK-BR-3 Exposure time: 180s.
Western blot analysis of various lysates, using CD31/PECAM1 Rabbit mAb (CAB19014) at 1:12500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using CD31/PECAM1 Rabbit mAb (CAB19014) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD31/PECAM1 Rabbit mAb (CAB19014) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using CD31/PECAM1 Rabbit mAb (CAB19014) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using CD31/PECAM1 Rabbit mAb (CAB19014) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human kidney tissue using CD31/PECAM1 Rabbit mAb (CAB19014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Mouse kidney tissue using CD31/PECAM1 Rabbit mAb (CAB19014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Mouse lung tissue using CD31/PECAM1 Rabbit mAb (CAB19014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat lung tissue using CD31/PECAM1 Rabbit mAb (CAB19014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
