The CD324/E-Cadherin Polyclonal Antibody (CAB24874) is a high-quality antibody developed for reliable detection and analysis of target proteins. E-cadherin is a calcium-dependent cell adhesion molecule encoded by CDH1, essential for establishing and maintaining epithelial cell morphology during embryogenesis and adulthood. Disruption of calcium binding causes embryonic lethality in mice due to failed blastocyst formation, and dysregulation of E-cadherin is strongly implicated in epithelial-to-mesenchymal transition and cancer progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CD324/E-Cadherin Polyclonal Antibody
SKU:
CAB24874
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8.
Purification Method
Affinity purification
Gene ID
12550
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CD324/E-Cadherin Rabbit pAb (CAB24874) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HeLa. Exposure time: 5s.
