The CD58 Monoclonal Antibody (CAB22276) is a high-quality antibody developed for reliable detection and analysis of target proteins. This cell surface glycoprotein is involved in T-cell co-stimulation and adhesion, crucial for immune responses. The antibody, generated in mice, is ideal for use in various research applications, including flow cytometry and immunohistochemistry.CD58 plays a vital role in immune cell interactions, facilitating the activation and regulation of T-cells. Its involvement in immune responses makes it a key target for studies in immunology, infectious diseases, and autoimmune disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD58 Monoclonal Antibody
SKU:
CAB22276
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC54834
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ag3, LFA3, LFA-3, CD58
Positive Sample:
MCF7
Cellular Localization:
Cell Membrane, Gpi-Anchor, Lipid-Anchor, Single-Pass Type I Membrane Protein.
Calculated MW:
28kDa
Observed MW:
70kDa
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
965
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD58 Rabbit mAb (CAB22276) at 1:100000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): A-204. Exposure time: 45 s.
