The CD58 Monoclonal Antibody (CAB22276) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD58 Monoclonal Antibody
SKU:
CAB22276
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC54834
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:50000 - 1:200000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ag3, LFA3, LFA-3, CD58
Positive Sample:
MCF7
Cellular Localization:
Cell Membrane, Gpi-Anchor, Lipid-Anchor, Single-Pass Type I Membrane Protein.
Calculated MW:
28 kDa
Observed MW:
70 kDa
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
965
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD58 Rabbit mAb (CAB22276) at 1:100000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): A-204. Exposure time: 45 s.
