The CD99 Polyclonal Antibody (CAB24663) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the CD99 protein, researchers can detect and analyze this molecule in a variety of cell types, making it a valuable asset for studies in immunology and cancer research.CD99 is a key player in immune response modulation, functioning as an inhibitory receptor that regulates inflammation and suppresses allergic reactions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD99 Polyclonal Antibody
SKU:
CAB24663
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MIC2, HBA71, MIC2X, MIC2Y, MSK5X, CD99
Positive Sample:
MOLT-4
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
19kDa
Observed MW:
32kDa
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.
Purification Method
Affinity purification
Gene ID
4267
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD99 Rabbit pAb (CAB24663) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC):CHO Exposure time: 90s.