The CDKN3 Antibody (CAB2061) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the CDKN3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal tool for studies in cell biology and cancer research.CDKN3 plays a crucial role in regulating cell division and promoting cell proliferation, making it a key player in the development and progression of cancer.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CDKN3 Antibody
SKU:
CAB2061
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MKPP SSIQ TSEF DSSD EEPI EDEQ TPIH ISWL SLSR VNCS QFLG LCAL PGCK FKDV RRNV QKDT EELK S
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:100 - 1:500
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KAP, CDI1, CIP2, KAP1, CDKN3
Positive Sample:
MCF7, Mouse liver
Cellular Localization:
Cytoplasm, Perinuclear Region.
Calculated MW:
24kDa
Observed MW:
24kDa/23kDa
The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1033
RRID
AB_2764084
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using CDKN3 Rabbit pAb (CAB2061) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Mouse liver using CDKN3 Rabbit pAb (CAB2061) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:60s.
