The CENPJ Polyclonal Antibody (CAB8721) is a vital tool for research involving the centromere protein J (CENPJ), a key player in centrosome maturation and spindle assembly during cell division. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the CENPJ protein, allowing for precise detection and analysis in various cell types.CENPJ is essential for proper cell division and is implicated in various diseases, including cancer and developmental disorders.
By studying the function of CENPJ, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets. The CENPJ Polyclonal Antibody is a valuable tool for investigating the role of CENPJ in these contexts and advancing our understanding of cellular processes critical for cell proliferation and genomic stability.
Product Name:
CENPJ Rabbit Polyclonal Antibody
SKU:
CAB8721
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1069-1338 of human CENPJ (NP_060921.3).
This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and cognitive disability. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
55835
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using CENPJ Rabbit pAb (CAB8721) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
